DNase I Chromatin Accessibility Analysis
نویسندگان
چکیده
منابع مشابه
Measuring Arabidopsis chromatin accessibility using DNase I-polymerase chain reaction and DNase I-chip assays.
DNA accessibility is an important layer of regulation of DNA-dependent processes. Methods that measure DNA accessibility at local and genome-wide scales have facilitated a rapid increase in the knowledge of chromatin architecture in animal and yeast systems. In contrast, much less is known about chromatin organization in plants. We developed a robust DNase I-polymerase chain reaction (PCR) prot...
متن کاملHigh-resolution analysis of c-fos chromatin accessibility using a novel DNase I-PCR assay.
In our previous study, c-fos chromatin accessibility was assayed using DNase I digestion and Southern blot analysis. This low-resolution mapping of c-fos chromatin accessibility demonstrated that serum stimulation of the c-fos enhancer induces a reversible increase in c-fos DNase I sensitivity and suggested that a 5' to 3' gradient of DNase I sensitivity may form downstream from the c-fos enhan...
متن کاملBreakthrough Technologies Measuring Arabidopsis Chromatin Accessibility Using DNase I-Polymerase Chain Reaction and DNase I-Chip Assays
Department of Biology and Zurich-Basel Plant Science Center, Eidgenössisch Technische Hochschule Zurich, CH–8092 Zurich, Switzerland (H.S., W.G., L.H.); Functional Genomics Center Zurich, University of Zurich/ Eidgenössisch Technische Hochschule Zurich, CH–8057 Zurich, Switzerland (W.G.); Department of Plant Biology and Forest Genetics, Uppsala BioCenter, Swedish University of Agricultural Scie...
متن کاملChromatin Accessibility Data Sets Show Bias Due to Sequence Specificity of the DNase I Enzyme
BACKGROUND DNase I is an enzyme which cuts duplex DNA at a rate that depends strongly upon its chromatin environment. In combination with high-throughput sequencing (HTS) technology, it can be used to infer genome-wide landscapes of open chromatin regions. Using this technology, systematic identification of hundreds of thousands of DNase I hypersensitive sites (DHS) per cell type has been possi...
متن کاملDifferential DNase I hypersensitivity reveals factor-dependent chromatin dynamics.
Transcription factor cistromes are highly cell-type specific. Chromatin accessibility, histone modifications, and nucleosome occupancy have all been found to play a role in defining these binding locations. Here, we show that hormone-induced DNase I hypersensitivity changes (ΔDHS) are highly predictive of androgen receptor (AR) and estrogen receptor 1 (ESR1) binding in prostate cancer and breas...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2019
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.3444